PTMScan Proteomics Lecture

The ability to identify and quantify changes in protein modification state levels in cell lines or tissue samples is critical for gaining insight into the biology and chemistry of biological systems. PTMScan® Proteomics Technologies, developed at Cell Signaling Technology, provide comprehensive analytical profiling of post-translational modifications (PTMs), including Ser, Thr, and Tyr phosphorylation, as well as acetylation, ubiquitination, and methylation. Discovery-mode studies, carried out with PTMScan® Discovery Technology, identify PTMs throughout the proteome in a variety of biological model systems and disease states. Alternatively, targeted-mode studies, performed using PTMScan® Direct Technology, allow for targeted screening of known PTMs on protein targets within a defined group of signaling pathways in response to a drug treatment or in the context of a specific disease state. Both technologies will be outlined with an emphasis on their direct application to drug discovery research.